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middle east respiratory syndrome related coronavirus mers cov s glycoprotein  (Sino Biological)


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    Structured Review

    Sino Biological middle east respiratory syndrome related coronavirus mers cov s glycoprotein
    Middle East Respiratory Syndrome Related Coronavirus Mers Cov S Glycoprotein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mers+cov+s/MERS-CoV+Spike%2FRBD+Protein+fragment/pmc12615495-44-17-31
    Average 93 stars, based on 34 article reviews
    middle east respiratory syndrome related coronavirus mers cov s glycoprotein - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: A colorimetric sandwich-type bioassay for SARS-CoV-2 using a hACE2-based affinity peptide pair
    Article Snippet: The conformation files were visually displayed in PyMol (v.2.0.4; https://pymol.org/2/ ) and data were analyzed using Origin (v.9.1; OriginLab, USA). .. The SARS-CoV-2 S _RBD -His recombinant protein (S _RBD ), SARS-CoV S _RBD -His recombinant protein, MERS-COV S _RBD -His recombinant protein, HCoV-NL63 S1-His recombinant protein and SARS-CoV-2 spike pseudovirus were obtained from Sino Biological, China. .. LY-13 lysis buffer purchased from AcroBiosystem, China.

    Expressing:

    Article Title: ACE2 engagement exposes the fusion peptide to pan-coronavirus neutralizing antibodies
    Article Snippet: .. For transient expression of HCoV spike proteins, 293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding HCoV spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS-CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation , using PEI in Optimem as above. .. For the SARS-CoV-2 S2P mutant, K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S).

    Article Title: ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies
    Article Snippet: .. Transient expression and monoclonal antibody staining of hCoV S-expressing HEK293T cells For transient expression of hCoV S proteins, HEK293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding hCoVs spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS- CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation (89), using PEI in Opti-MEM as above. .. For the SARS-CoV-2 S2P mutant, K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S).

    Plasmid Preparation:

    Article Title: ACE2 engagement exposes the fusion peptide to pan-coronavirus neutralizing antibodies
    Article Snippet: .. For transient expression of HCoV spike proteins, 293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding HCoV spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS-CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation , using PEI in Optimem as above. .. For the SARS-CoV-2 S2P mutant, K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S).

    Article Title: Identification of bis-benzylisoquinoline alkaloids as SARS-CoV-2 entry inhibitors from a library of natural products in vitro
    Article Snippet: .. SARS-CoV S-expressing plasmid (Cat: VG40150-ACGLN, named as pS-SARS) and MERS-CoV S-expressing plasmid (Cat: VG40069-CF, named as pS-MERS) were obtained from Sino Biological Inc (Beijing, China). ..

    Article Title: ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies
    Article Snippet: .. Transient expression and monoclonal antibody staining of hCoV S-expressing HEK293T cells For transient expression of hCoV S proteins, HEK293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding hCoVs spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS- CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation (89), using PEI in Opti-MEM as above. .. For the SARS-CoV-2 S2P mutant, K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S).

    Clone Assay:

    Article Title: ACE2 engagement exposes the fusion peptide to pan-coronavirus neutralizing antibodies
    Article Snippet: .. For transient expression of HCoV spike proteins, 293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding HCoV spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS-CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation , using PEI in Optimem as above. .. For the SARS-CoV-2 S2P mutant, K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S).

    Article Title: ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies
    Article Snippet: .. Transient expression and monoclonal antibody staining of hCoV S-expressing HEK293T cells For transient expression of hCoV S proteins, HEK293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding hCoVs spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS- CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation (89), using PEI in Opti-MEM as above. .. For the SARS-CoV-2 S2P mutant, K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S).

    Membrane:

    Article Title: Multivalent vaccines for rabies virus and coronaviruses
    Article Snippet: .. The nitrocellulose membrane was then blocked in TBST (100 mM Tris-HCl pH 7.9, 150 mM NaCl, 0.01% Tween20) containing 5% (w/v) non-fat dried milk at room temperature for 1 h. After blocking, the membrane was incubated overnight with rabbit antisera specific for the RABV glycoprotein (kindly provided by Dr. Dietzschold, Thomas Jefferson University) or rabbit serum against the S1 or S2 subunit of MERS-CoV S (Sinobiological) at a dilution of 1:1000 in PBS containing 5% BSA. ..

    Blocking Assay:

    Article Title: Multivalent vaccines for rabies virus and coronaviruses
    Article Snippet: .. The nitrocellulose membrane was then blocked in TBST (100 mM Tris-HCl pH 7.9, 150 mM NaCl, 0.01% Tween20) containing 5% (w/v) non-fat dried milk at room temperature for 1 h. After blocking, the membrane was incubated overnight with rabbit antisera specific for the RABV glycoprotein (kindly provided by Dr. Dietzschold, Thomas Jefferson University) or rabbit serum against the S1 or S2 subunit of MERS-CoV S (Sinobiological) at a dilution of 1:1000 in PBS containing 5% BSA. ..

    Incubation:

    Article Title: Multivalent vaccines for rabies virus and coronaviruses
    Article Snippet: .. The nitrocellulose membrane was then blocked in TBST (100 mM Tris-HCl pH 7.9, 150 mM NaCl, 0.01% Tween20) containing 5% (w/v) non-fat dried milk at room temperature for 1 h. After blocking, the membrane was incubated overnight with rabbit antisera specific for the RABV glycoprotein (kindly provided by Dr. Dietzschold, Thomas Jefferson University) or rabbit serum against the S1 or S2 subunit of MERS-CoV S (Sinobiological) at a dilution of 1:1000 in PBS containing 5% BSA. ..

    Staining:

    Article Title: ACE2-binding exposes the SARS-CoV-2 fusion peptide to broadly neutralizing coronavirus antibodies
    Article Snippet: .. Transient expression and monoclonal antibody staining of hCoV S-expressing HEK293T cells For transient expression of hCoV S proteins, HEK293T cells were co-transfected, with plasmid encoding ZsGreen (Bei Resources, catalog no. NR-52516) and corresponding hCoVs spike proteins: SARS-CoV-2 Wuhan-Hu-1 S (catalog no. NR-52514) from Bei Resources; MERS- CoV S (VG40069-G-N), 229E S (VG40605-UT), NL63 S (VG40604-UT) from SinoBiological; SARS-CoV S (VG40150-G-N) from SinoBiological that was cloned into pHDM expression plasmid with 19 amino-acid C-terminal truncation (89), using PEI in Opti-MEM as above. .. For the SARS-CoV-2 S2P mutant, K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S).



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